Complete Genomics Inc., a world life science instruments chief and subsidiary of MGI Tech Co., Ltd. (“MGI”), as we speak launched new revolutionary merchandise and costs at Advances in Genome Biology and Technology (AGBT) General Meeting.
Complete Genomics presents T Series, G Series, and E Series genetic sequencers overlaying big selection from low- medium- high- extremely excessive throughput sequencing purposes. “We have made it our mission to address the global need for faster, cost-effective, and accurate sequencing whether it be to identify and trace pathogens in today’s post-pandemic world or to meet the promise of precision medicine.” mentioned Rade Drmanac, PhD, Chief Scientific Officer.
Image Credit: Complete Genomics
DNBSEQ-T20×2, Breaks the Boundaries and Reads the Sequence of Whole Human Genome for Sub $100
DNBSEQ-T20×2 represents a T-series product evolution designed for big inhabitants genome initiatives. Using an open-type massive sequencing slide minimize from a complete silicon wafer, one DNBSEQ-T20×2 helps the operation of 6 slides concurrently, producing as much as 42Tb per run with PE100 and even 72Tb per run with PE150 in a smaller footprint than DNBSEQ-T10×4*.
Enabled by modern dip-immersion biochemistry, DNBSEQ-T20×2 perfects the unthinkable course of for consecutive robotic dipping of a number of slides in the identical reagent. Immersion fluidics on silicon slides with out cowl permits very uniform response over all the floor of those massive slides guaranteeing excessive sequencing high quality, largest throughput and lowest price. A single set of DNBSEQ-T20×2 can produce as much as 50,000 WGS per yr for underneath $100 per 30x human genome together with instrument depreciation.
In specific, DNBSEQ-T20×2 is supplied with 2 imagers and a rotational robotic arm dealing with 6 slides delivering 72Tb/run to WGS, WGBS, WES, RNA-seq, single cell, STOmics(a big scale Spatial Omics instrument with nanoscale decision), and different sequencing purposes, together with pooling and sequencing totally different libraries concurrently. “The commercialization of DNBSEQ-T20x2 will become an important force driving the development and expansion of the global genetic industry and accelerate the progress of the human genome understanding and medical applications.” mentioned Dr. Drmanac. “Now many more samples can be sequenced or a deeper more informative and accurate sequencing can be afforded per sample”.
Since the low consumable price is achieved by working concurrently 6 slides at or close to its working capability, DNBSEQ-T20×2 have to be ordered with sure annual minimal quantity of consumables. DNBSEQ-T20×2 might be commercially obtainable within the US in Q3 2023, and in China in Q2 2023.
During the convention, Complete Genomics additionally introduced a surprising new US reagent promotional worth of $1.5/Gb for its flagship sequencer DNBSEQ-T7 that’s already available on the market. The new DNBSEQ-T7 reagent worth permits quick entry to a $150 genome at excessive DNBSEQ high quality even when solely a dozen of samples is sequenced per run. Additionally, large-scale, inexpensive sequencing initiatives like STOmics can begin instantly utilizing a number of DNBSEQ-T7 sequencers per lab. A set of three DNBSEQ-T7 sequencers gives close to 50,000 30x hWGS per yr.
DNBSEQ-G99, Unleashes Your Ultimate Sequencing Speed with Each Run (PE150) < 12 hrs
DNBSEQ-G99, as the newest mannequin of the G Series, is an ultra-high velocity gene sequencer providing mid- to low- vary throughput making it a superior choice for focused gene sequencing and small genome sequencing. DNBSEQ-G99 for the primary time adopts triangular matrix sign spots on sequencing stream cell, reaching increased density of information output, with an total throughput of 8-48 Gb per run. In addition, geared up with proprietary high-performance optical tools and high-precision temperature management system, the turnaround time of PE150 sequencing run knowledge has been shortened to inside 12 hours. Useful shorter learn studies can be found even inside first 2.5h throughout PE100 or PE150 sequencing runs.
DNBSEQ-G99 was instrumental within the discovery of the primary imported case of Monkeypox in Chongqing, China. The virus pressure was shortly recognized by the entire genome sequence accomplished by DNBSEQ-G99. Three totally different read-length metagenome sequencing statistics of Monkeypox virus-specific reads accounted for about 0.35% on common in qPCR testing for pattern with Ct worth of roughly 21. Results confirmed the common depth of the Monkeypox virus genome was roughly 35X on the learn size of SE40, 110X at SE100, and 200X at PE100, with greater than 99.03% protection above 100X. Meanwhile, the multi-point knowledge output mode of DNBSEQ-G99 (bioanalysis by sequencing, BBS) shortened the supply time to inside 2.5 hours with out limiting the ultimate sequencing learn size making it the only option for pressing and a number of testing eventualities in a public well being system. The bioinformatics by sequencing (BBS) mode was utilized in Monkeypox virus tracing ensuing within the first batch of uncooked reads outcomes delivered in solely 9 hours.
As we transfer from a one-size-fits-all method right into a precision medication method focusing on the precise therapies to the precise sufferers on the proper time, the velocity required to determine the genetic make-up of the person affected person has grow to be important. DNBSEQ-G99 addresses that important want with its speedy and high-quality knowledge output, and improves the turnaround instances for clinically related biomarkers. DNBSEQ-G99 is very appropriate for precision oncology, tumor detection in hospital purposes, and decentralized researches, and it opens a brand new alternative for labs to adapt to those adjustments and add worth to their choices.
At current, DNBSEQ-G99 helps sequencing reads lengths together with PE50, SE100, and PE150, whereas reagent kits with longer reads lengths similar to SE400 and PE300 might be obtainable sooner or later. DNBSEQ-G99 is deliberate to enter the US market in Q2, 2023.
Image Credit: Complete Genomics
stLFR Library Prep Kit, a Single-Tube Process for Unique Read Co-barcoding in Long DNA Fragments
Complete Genomics gives a number of learn lengths choices. stLFR (single tube lengthy fragment reads) is predicated on patented DNA co-barcoding know-how to offer artificial or linked lengthy reads on DNBSEQ platforms. This instantly obtainable equipment overcomes the constraints of quick learn lengths and permits prime quality small and huge structural variant calling, haplotype phasing of over 99% of the human genome, de novo meeting, and different lengthy fragment purposes. stLFR kits mixed with PCR-free WGS allow extra full and extra correct haplotype-phased genomes at inexpensive worth resulting in a brand new WGS commonplace for each analysis and medical purposes.
Complete Genomics, an modern sequencing methods firm, is now providing six sequencers to US sequencing labs together with transportable sequencer DNBSEQ-E25*, and excessive throughput sequencer DNBSEQ-T10x4, and medium throughput benchtop sequencer DNBSEQ-G400*. Complete Genomics additionally gives pattern and library prep automation and downstream bioinformatics options.